Skip to main content
. 2011 Oct;90(4):643–651. doi: 10.1189/jlb.0111043

Figure 5. Gel-shift analysis of proteins in myeloid extracts using C/EBP, AP-1, or hybrid sites.

Figure 5.

(A) Nuclear extracts (12 μg) from HF-1 cells cultured in G-CSF for 1 day were subject to gel-shift/super-shift assay using the αα- or αJ-radiolabeled probes. Antisera used were normal rabbit Ig (Ig), C/EBPα (α), C/EBPβ (β), C/EBPε (ε), c-Jun (cJ), JunB (JB), or c-Fos (cF). Arrows indicate super-shift complexes. Positions of C/EBPα:C/EBPα and C/EBPβ:C/EBPβ gel-shift complexes are also indicated (α and β). (B) Nuclear extracts (12 μg) from HL-60 cells cultured in PMA for 6 h were subjected similarly to gel-shift/super-shift assay using the αα-, αJ-, and JJ-radiolabeled probes.