Figure 6. Oligonucleotide pull-down analysis of proteins in myeloid extracts using C/EBP, AP-1, or hybrid sites.

(A) Nuclear extracts prepared from HF-1 cells cultured with G-CSF (G) for 24 h or from noninduced HF-1 cells (NI) were incubated with biotinylated αα, αJ, or JJ oligonucleotides, followed by streptavidin beads, washed, and subjected to Western blotting for the indicated proteins. (B) Similar analysis was conducted using nuclear extracts from HL-60 cells cultured with PMA for 6 h or from noninduced HL-60 cells. Input samples represent 10% of the extract used for oligonucleotide pull-down.