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. 2011 Aug 5;4:15. doi: 10.1186/1756-8935-4-15

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Copyright ©2011 Gréen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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High-performance capillary electrophoresis (HPCE) separations of H1 histones and reversed phase high performance liquid chromatography (RP-HPLC)-fractionated H1.5 (inset) from (A) activated T cells, and (B) exponentially growing Jurkat cells. The peaks were identified as: (1), unphosphorylated H1.5; (2) unphosphorylated H1.4; (3) monophosphorylated H1.5; (4) monophosphorylated H1.4; (5) unphosphorylated H1.3; (6) diphosphorylated H1.5, together with unphosphorylated H1.2 and possibly diphosphorylated H1.4; (7) monophosphorylated H1.3; and (8) monophosphorylated H1.2 together with triphosphorylated H1.5.