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Zymogram assay of the second batch culture. Fractions P2-1 and P2-2 in Figure 2 corresponding to 52 to 61 mL and 64 to 73 mL were pooled and then separated on 8% and 12% SDS-PAGE-based zymogram assays, respectively. Twelve bands were recovered and analyzed for possible glucanase sequences using ESI-Trap LC-MS/MS.
