Table 2.

Summary of transcriptome analyses^a

Transcriptome analyses	W5 cDNA
Roche 454 GS FLX
Run cycles Total number of reads Total read length, Mb	1 526,516 111
Average read length, bp	210.2
Illumina GA II Paired-End
Read length, bp	40 + 40
Run lanes	1
Total number of reads Total read length, Mb	14,802,234 1,184
GC content	37.1%
Contig assembly
Contig numbers	20,232
Total contig lengths, Mb	9.0
Average contig length, bp	447.2
Largest contig length, bp	4,397
Gene prediction, length > 500 bp
Number of ORFs	2,284
Number of domains predicted in ORFs	9,720
Average ORF length, bp	846.9
Maximum RPKMb value	31,647.2
Number of GH-like contigs	219

^aGC: the nucleotide G+C % ; GH: glycosyl hydrolase; ORF: open reading frame; RPKM: Reads Per Kilobase of exon model per Million mapped reads. ^bRPKM is a method for estimating relative expression levels of a transcriptome by normalizing short-read expressed tags from deep sequencing over ORF length and total reads (RPKM) [35].