Table 2.
Primer pairs developed in this study and variability of MLSA loci among 25 strains analysed
| Locus | Primer name | Primer sequence (5'-3') | Annealing T° (°C) |
Amplicona (bp) |
Locus sequence b (bp) |
Variable sites | Sequence types |
|---|---|---|---|---|---|---|---|
| Loc-01 | MLSA-Mccp-01-F | GCTTATAGTGTTGTTGATACG | 53 | 694 | 590 | 5 | 5 |
| MLSA-Mccp-01-R | GCAATAATCAATTAGCACAG | ||||||
| Loc-03 | MLSA-Mccp-03-F | ATTCCTCTCATTGAAGTTAC | 47 | 765 | 664 | 7 | 7 |
| MLSA-Mccp-03-R | TAGATTAAGAGTCACAATGC | ||||||
| Loc-11 | MLSA-Mccp-11-F | TGATGGAATTATGTGTAGAGC | 53 | 746 | 637 | 6 | 6 |
| MLSA-Mccp-11-R | ATGAACGATCTTGATGTTCC | ||||||
| Loc-12 | MLSA-Mccp-12-F | GGTATGGAGTTGATTTTGAAAC | 58 | 742 | 646 | 4 | 4 |
| MLSA-Mccp-12-R | GCTCCAGCTAAAGCATTATTA | ||||||
| Loc-15 | MLSA-Mccp-15-F | GGACGAATTTATTTAGTTTCTGCTG | 58 | 802 | 691 | 4 | 4 |
| MLSA-Mccp-15-R | ACATTAGTTTGCATACCACCAGTAA | ||||||
| Loc-17 | MLSA-Mccp-17-F | TAAACCAGAGCAAAACGGTA | 58 | 751 | 649 | 8 | 6 |
| MLSA-Mccp-17-R | AACACTAACAATTCCAACAGC | ||||||
| Loc-20 | MLSA-Mccp-20-F | CTAGTTAATTTTGGAGCCGA | 53 | 781 | 696 | 7 | 7 |
| MLSA-Mccp-20-R | CATCAATTGTTGATGAATCG | ||||||
| H2c | N/A | N/A | N/A | N/A | 2174 | 12 | 8 |
| 8 concatenated loci | N/A | N/A | N/A | N/A | 6747 | 53 | 15 |
aAmplicon sizes in base pairs correspond to F38T amplifications
bLocus sequence sizes in base pairs correspond to F38T data
cFor information regarding H2 locus amplification and sequencing refer to [8]
N/A: does not apply.