Table 1. Enzymatic characteristics of selected oral microorganisms cultured on gluten agar.
| Aer/Anaer | Strain ID | GenBank Accession | Strain namea | Hydrolysis of gluten-based substratesb | |||
| YPQ | QQP | PPF | PFP | ||||
| Aerobic | WSA-2B | JF895494 | Rothia mucilaginosa HOT-681 | +++ | − | − | − |
| WSA-7A | JF895495 | Streptococcus mitis HOT-677 | +/− | − | − | − | |
| WSA-8 | JF895496 | Rothia aeria HOT-188 | +++ | − | − | − | |
| WSA-10 | JF895497 | Staphylococcus epidermidis HOT-601 | − | − | − | − | |
| WSA-26 | JF895498 | Rothia mucilaginosa HOT-681 | +++ | − | − | − | |
| Anaerobic | WSAN-14 | JF895499 | Bifidobacterium longum HOT-B54 | − | − | − | − |
| WSAN-16 | JF895500 | Bifidobacterium longum HOT-B54 | − | − | − | − | |
| WSAN-24 | JF895501 | Bifidobacterium longum HOT-B54 | − | − | − | − | |
| WSAN-25 | JF895502 | Veilonella atypica HOT-524 | − | − | − | − | |
| PAN-0 | JF895503 | Streptococcus pneumoniae HOT-734 | − | − | − | − | |
| PAN-5 | JF895504 | Bifidobacterium dentium HOT-588 | − | − | − | − | |
| PAN-8 | JF895505 | Bifidobacterium dentium HOT-588 | − | − | − | − | |
| PAN-18 | JF895506 | Bifidobacterium dentium HOT-588 | − | − | − | − | |
| PAN-19 | JF895507 | Bifidobacterium dentium HOT-588 | − | − | − | − | |
| PAN-23 | JF895508 | Bifidobacterium dentium HOT-588 | − | − | − | − | |
| Aer+Anaer | Whole plaque | Mixture of bacteria | +++ | +++ | +++ | +++ | |
a
Speciation was carried out by partial 16S rDNA gene sequencing. All sequences were greater than 99% similar reference sequences at the Human Oral Microbiome Database (www.homd.org).
b
Substrates (final concentration 200 µM) were mixed with bacterial suspensions in saliva ion buffer (OD620 1.2). Hydrolysis was measured spectrophotometrically at 405 nm after 24 h incubation.