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Effect of additions of Pi and ADP alone to State 4 porcine heart mitochondria. A) ADP added to State 4 mitochondria at the time indicated. The addition of Pi then initiated oxidative phosphorylation and associated oxygen consumption. B) Addition of Pi to State 4 mitochondria at the time indicated. Addition of ADP then initiated oxidative phosphorylation and associated oxygen consumption. Data is adapted from 133.
