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. 2011 Sep 21;6(9):e24874. doi: 10.1371/journal.pone.0024874

Figure 3. Spiking activity regulates ST8SiaII, but not ST8SiaIV, mRNA expression levels in cortical organotypic cultures.

Figure 3

ST8SiaII (A) but not ST8SiaIV (B), developmental downregulation is impaired by TTX application (Two- Way ANOVA, Dunn's test p<0.05 for ST8SiaII in culture treated with TTX from EP8-14 and EP11-14; p>0.05 for ST8SiaIV). Conversely, increasing spiking activity levels by BMI treatment accelerates ST8SiaII (A; Two-Way ANOVA, Dunn's test p<0.05), but not ST8SiaIV (B; p>0.05) mRNA level downregulation. ST8SiaII and ST8SiaIV of each sample are first normalized to GAPDH levels, then to age-matched controls from the same litters and experiment. Graphs are plotted as explained in Figure 2.