Table 2. Microsatellites markers selected for the LOH analysis.
Markers | Region Chromosome | Size (bp) | Localization (bp) | Sequence | |
D7S527 | 7q21.3 | 273–297 | 95453048 | Forward | CATTGCAAACTCAGGAGATA |
Reverse | TAACAGAGGCATGAAAACCA | ||||
D7S662 | 7q22.1 | 204–234 | 100950585 | Forward | GTTGACAGACAAGCACAGAC |
Reverse | AGCTGTTTCCCATTTCCA | ||||
D7S2536 | 7q22.1 | 118–141 | 101235892 | Forward | ACACTCCGCCACCTTG |
Reverse | CAACAACTGTTCCTAAAGCCT | ||||
D7S515 | 7q22.1 | 128–190 | 101490495 | Forward | GGGAGTTACTACCCTCACTTAATG |
Reverse | GGACTGGGCAGCAAAG | ||||
D7S518 | 7q22.1 | 179–201 | 101648938 | Forward | CAGTAGGCAGGGGTGG |
Reverse | GGGTGTGTCTGTGTGACAAC | ||||
D7S500 | 7q33 | 188–210 | 134759259 | Forward | CCAGAATTGAAAACTCAGCA |
Reverse | ATTGATTGAGGAACTGAACTTACCT | ||||
D7S550 | 7q36.3 | 177–200 | 155210270 | Forward | TCTCATCTGTGAATGCACTATC |
Reverse | GCAGTTGGGTTATTTCAAGTC |