Table 5.

Effect of weaning induced stress on the relative gene expression of toll-like receptor (TLR) 4, glucocorticoid receptor (GR)α, nuclear factor kappa B (NFκB), Fas, p21, CD62l, haptoglobin and bactericidal/permeability increasing protein (BPI) in weaned beef calves.

	Gender		Location		s.e.	Time				s.e.	P-Values
Variable	Male	Female	Near	Away		0	1	3	7		G	L	T	I
TLR4	9.4	7.5	8.0	8.9	1.4	5.2	8.3b	8.9c	11.5c	1.3	NS	NS	***	NS
GRα	13.4	16.5	16.4	13.5	2.1	6.4	19.8c	16.9c	16.6c	2.5	NS	NS	***	NS
NFκB1	2.9	2.9	2.8	3.0	0.27	2.5	3.1b	2.9	3.1b	0.25	NS	NS	*	NS
NFκB2	2.9	2.8	2.9	2.7	0.22	2.8	2.9	2.7	2.8	0.24	NS	NS	NS	NS
Fas	27.9	24.5	25.7	26.8	4.8	9.7	33.6c	26.4c	35.2c	5.1	NS	NS	***	NS
p21	5.8	5.5	5.4	5.9	0.70	6.2	4.9	5.7	5.6	0.71	NS	NS	NS	NS
CD62L	33.7	35.5	34.4	34.8	5.1	26.0	34.8b	37.5c	40.1c	4.3	NS	NS	***	NS
Haptoglobin	14.4	16.6	13.7	17.3	3.9	12.8	16.3	17.7	15.2	3.5	NS	NS	NS	NS
BPI	14.8	15.6	16.5	13.9	2.5	18.3	16.7	14.8a	11.1c	2.1	NS	NS	***	NS

The values are expressed as least squares means (Lsmeans) and s.e.

G = gender, L = location, T = time, I = interaction, NS = not significant (P > 0.05). * = P < 0.05, ** = P < 0.01, *** = P < 0.001.

^{a, b, c}Within rows, Lsmeans differ from pre-weaning baseline by P < 0.05, P < 0.01 and P < 0.001, respectively.