Figure 3. Cell-cell contact is necessary for activated microglia-induced neurite outgrowth inhibition and growth cone collapse.

A, Results of neurite outgrowth assay across 4 treatment groups (neurons-only, neurons + LPS, neurons co-cultured with unactivated microglia, neurons co-cultured with activated microglia) in the transwell system (n = 3). The neurons, seeded to the lower plane, were separately co-cultured with the unactivated and activated primary microglia, seeded to the upper plane. B, Results of growth cone collapse assay across 4 treatment groups (neurons-only, neurons + LPS, neurons co-cultured with unactivated or activated microglia) in the transwell system (n = 3). Data are represented as mean ± SEM.