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. 1989 May 11;17(9):3479–3490. doi: 10.1093/nar/17.9.3479

Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.

M Ohki 1, C L Smith 1
PMCID: PMC317790  PMID: 2657661

Abstract

The location of chromosomal DNA replication forks was identified in synchronously replicating E. coli cultures by pulse labeling DNA at specific times with 14C-thymidine and following incorporation of radionucleotide into genomic Not I restriction fragments. This technique could be used to characterize chromosomal DNA replication, to characterize mutations which affect this process, to identify the location of DNA replication origins and termini as well as aid in the construction of macrorestriction maps. Here, we further characterize the DNA replication mutations divE and dnaK and preliminary characterize the genomic organization of E. coli isolate 15.

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Selected References

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