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. 2011 Sep 21;6(9):e25269. doi: 10.1371/journal.pone.0025269

Figure 6. Liraglutide treatment reduces steatosis and ER stress in high fat diet fed mice.

Figure 6

Mice were fed American Lifestyle-Induced Obesity Syndrome (ALIOS) diet and high fructose corn syrup for 8 weeks. One set of animals was subsequently treated with 200 µg/kg body weight liraglutide for next 4 weeks while maintaining other on high fat high sucrose diet. (A). Oil Red O staining of liver sections from mice kept on normal chow and treated with saline [C], liraglutide [L] and those given ALIOS diet alone [A] and subsequently liraglutide [AL]. (B) Immunoblotting of mouse liver lysates for GRP78 and CHOP. (C) Densitometric analysis of immunoblot (C: control, L: liraglutide treated, A: ALIOS fed, A+L: ALIOS fed and then liraglutide injected). (D) Real time mRNA quantification of GRP78 and CHOP. (E) Immunohistochemical staining of mouse liver sections for GRP78 and CHOP. All studies were conducted at least three times in triplicate with fresh lysates, *p<0.05 Student's t-test.