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Hepcidin/pro-hepcidin was detected by immunohistochemistry. Hepcidin/pro-hepcidin positive staining was significantly increased in cerebral cortex, hippocampus and corpus striatum of ischemic brain. The mean density of positive staining was measured by Image-Pro Plus soft. Data are presented as means ±SEM, n = 3. *p<0.05, vs. contralateral side.
