Table 2.
In vitro development potential and quality of bovine presumed zygotes
| Culture system | Presumed zygote (n) | % cleaved (range in %) |
% blastocyst (range in %) |
Total blastomeres (range) |
% apoptotic blastomeres (range in %) |
|---|---|---|---|---|---|
| ControlOPU | 201 | 64.3a ± 32.0 (0-100) | 13.9a ± 18.1 (0-57.1) | 118.3a ± 24.4 (65-163) | 4.6a ± 2.0 (1.3-7.5) |
| Agar OPUx | 151 | 82.8a± 26.1 (0-100) | 35.2b ± 26.3 (0-100) | 128.2a ± 29.5 (73-215) | 4.9a ± 1.6 (2.1-7.8) |
| s IVPy | 120 | 74.0a ± 11.6 (53.3-100) | 30.8b ± 13.8 (0-50.0) | 123.1a ± 28.9(65-188) | 3.8a ± 2.7 (1-10.8) |
| dmapIVPz | 143 | 72.8a ± 5.0 (67.3-76.9) | 28.6b ± 3.9 (25.0-32.7) | 124.2a ± 36.7 (65-175) | 5.3a ± 2.8 (1.2-11.0) |
Cleavage and blastocyst development rates were calculated based on total presumed zygotes transferred into IVC medium.
a, b Values with different superscript letters within a column differ significantly (P < 0.01).
x Development rates are those of OPU cultured zygotes. The development rates of agar-embedded slaughterhouse embryos were not considered.
y A group of 10 COCs from slaughterhouse ovaries was used for IVP.
z Slaughterhouse COCs pre-treated with 2 mM 6-dimethylaminopurine for 6~7 h before maturation followed by IVM, IVF and IVC.