Figure 6. Hoxa1^WT and Hoxa1^I-V relieve MCF7 cells from contact inhibition, while expressing Hoxa1^WM-AA does not.

MCF7 cells were transiently transfected for Hoxa1^WT, Hoxa1^I-V and Hoxa1^WM-AA, together with Pbx1a and Prep1 cofactors, and grown for three weeks. Controls included cells transfected for the potent oncogene hRAS or cells transfected for Pbx1a and Prep1 only. Foci formation was observed for hRAS, Hoxa1^WT and Hoxa1^I-V transfected cells (arrowheads) while not for CTL and Hoxa1^WM-AA cells.