Fig. 3.

SEM and TEM images of HL60 cells treated with NRC-03. (A–F) Treatment was carried out for 0 (A,B), 1 (C,D) and 5 (E,F) minutes with 32 μg/ml NRC-03. Note the loss of microvilli and appearance of membrane blebs (white arrows) and membrane damage (black arrow) after 1 minute, and disruption of membrane after 5 minutes. (G–L) TEM images of HL60 cells treated with NRC-03 for 0 (G,H), 1 (I,J) and 5 (K,L) minutes. Black arrows in G, I and K indicate cells shown at high magnification in plates H, J and L. (M–P) SEM images of HMECs treated for 0 (M,O) or 4 (N,P) hours with 32 μg/ml NRC-03 do not show membrane blebs or damage after treatment, although some cell shrinkage and rounding is evident.