Table 1.
Primer sequences used for quantitative real-time polymerase chain reaction (qRT-PCR)
| Primer | Forward sequence | Reverse sequence |
|---|---|---|
| GAPDH | CCAGGTTGTCTCCTGCGACT | ATACCAGGAAATGAGCTTGACAAAGT |
| PPARα | TGCTGGTATCGGCTCAATAA | TCCTGCCACTTGCTCACTAC |
| CYP2E1 | CCATCGGCACCATGGCGGTT | GCCCGAAGCGCTTTGCCAAC |
| F4/80 | CTTTGGCTATGGGCTTCCAGTC | CAAGGAGGACAGAGTTTATCGTG |
| CD3 | AGAGCAGCTGGCAAAGGTGGTGTC | CAGCCATGGTGCCCGAGTCTAGC |
| CD4 | TCACCTGGAAGTTCTCTGACC | GGAATCAAAACGATCAAACTGCG |
| CD8 | CTCTGGCTGGTCTTCAGTATGA | TCTTTGCCGTATGGTTGGTTT |
| TNFα | ACGGCATGGATCTCAAAGAC | CGGACTCCGCAAAGTCTAAG |
| IL-6 | GGCCTTCCCTACTTCACAAG | ATTTCCACGATTTCCCAGAG |
| IL-4 | AGGTCACAGGAGAAGGGACGCC | TGCGAAGCACCTTGGAAGCCC |
| IL-13 | TGTTTCGCCACGGCCCCTTC | TGCTCAAGCTGCTGCCTGCC |
| IFNγ | ATGAACGCTACACACTGCATC | CCATCCTTTTGCCAGTTCCTC |
| TGFβ | CAACAATTCCTGGCGTTACCTTGG | GAAAGCCCTGTATTCCGTCTCCTT |
Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PPARα, peroxisome proliferator-activated receptor α; CYP2E1, cytochrome p450 enzyme; TNFα, tumor necrosis factor-α; TGFβ, transforming growth factor-β; IL, interleukins; IFNγ, interferon gamma; TGFβ, transforming growth factor-β.