Table 4.

Effect of HF intake on hepatic expression of selected genes related to lipid catabolism in Cc1^+/+ and Cc1^−/− mice

	Cc1+/+		Cc1−/−
	RD	HF	RD	HF
PPARα	5.57 ± 0.64	9.50 ± 0.33a	8.50 ± 0.37b	10.9 ± 0.67a
CYP2E1	555.0 ± 77.0	559.0 ± 70.0	500.0 ± 38.4	817.0 ± 136.0a

Notes: Male mice (n > 7; 3 months of age) were fed RD or HF for 3 months. Mice were fasted overnight, the tissues removed and gene expression assessed by semiquantitative real time-PCR analysis. Values are normalized to GAPDH and expressed as mean units ± SEM.

^aP < 0.05 HF vs RD;

^bP < 0.05 Cc1^−/− vs Cc1^+/+ in the same feeding group.

Abbreviations: Cc1^−/−, global ceacam1 null mouse; Cc1^+/+, wild-type mouse from the same genetic background as Cc1^−/− mice; RD, regular diet; HF, high-fat diet; PPARα, peroxisome proliferator-activated receptor α; CYP2E1, cytochrome p450 enzyme.