FIG. 5.

DAF-displaying Ads trigger reduced humoral transgene (HIV-Gag)–specific adaptive immune responses when administered IM into Ad5-naive mice. Plasma from naive (n = 3), conventional Ad5 (n = 4), or retro-DAF-displaying Ad5-treated (n = 4) BACB/c mice was collected at 14 dpi, and Ad5-specific or HIV-Gag–specific total IgG levels were measured as detailed in Materials and Methods. The error bars represent ± SD. Statistical analysis was completed using two-tailed Student's t test to compare two groups of virus-injected animals (^#p < 0.05, ^##p < 0.001) or to compare each of Ad5-injected group with naive (*p < 0.05, **p < 0.01). No significant Ad5-specific IgG was detected in any group. Significant (1:10) titers of Gag-specific IgG were detected in DAF-Ad5-Gag–treated mice; significant (up to 1:200) titers of specific IgG were detected in Ad5-Gag–treated mice.