FIG. 4.

Time course of transgene expression in the livers of mice. Each group of mice was injected with 5×10¹² vp/kg HDAdLacZ. (a) X-Gal staining of livers from WT, NOD2–/–, MyD88–/–, and MyD88–/–;NOD2–/– mice was performed both 3 days and 3 weeks post injection. The animals were sacrificed at 3 days and 3 weeks post injection, and β-Gal activity of each sample was measured at the same time points (b) (*p<0.001). The copy numbers of vector genome DNA (c) and β-Gal mRNA transcripts (d) in livers were also determined by real-time quantitative PCR (*p<0.002 for both c and d). Data are presented as means±SD (n=4). Color images available online at www.liebertonline.com/hum