FIG. 5.

Transgene expression is partially regulated by NOD2 signaling independent of epigenetic modification in vitro. (a) BMDMs derived from WT or NOD2–/– mice were infected with 5,000 vp/cell HDAdLacZ for 3 hr. Cells were harvested at 24 hr and were evaluated by real-time quantitative PCR for vector genome DNA and β-Gal mRNA copy numbers. Data are presented as means ± SD (n = 4). The experiments were repeated with similar results. *p < 0.02. (b) Schematic structure of the HDAdLacZ vector genome and location of primer pair used for quantification of ChIP data using real-time quantitative PCR. (c) BMDMs derived from WT and NOD2–/– were infected with 5,000 vp/cell HDAdLacZ for 3 hr. Cells were harvested at 24 hr and ChIP experiments were performed as described in Materials and Methods. Ratio of vector DNA associated with Ac-K9-H3 to Met-K9-H3 was calculated. A higher ratio correlates with transcriptionally active loci. Data are presented as means ± SD (n = 5).