FIG. 1.

CMV, CBh, and CBA promoters demonstrate distinct kinetics in cultured rat hippocampal slices. Rat hippocampal slices were infected with 5×10⁷ VG after 7 days in culture, and GFP expression was monitored for 5 days. The time course of GFP expression was examined for the CMV (A–E), CBh (F–J), and CBA (K–O) promoters at five time points. All images are mapped to the pseudo-color gray wedge depicted in (O). All images are set to equivalent scales with the scale bar in (O) set to 600 μm. All three promoters demonstrated observable fluorescence 24 hr after infection and steadily increased during the 5-day time course (P). The fluorescence intensity for each measurement was normalized to a fluorescent test strip recorded each day. The values are scaled to account for exposure time required for each image. Note: The exposure times were adjusted to ensure all images were acquired within the dynamic range of the detector, and the detector linearity was evaluate each day for the entire time course (P). Color images available online at www.liebertonline.com/hum