FIG. 6.
In vivo expression of the MeP promoter at 14 weeks in the CNS. Adult mice were injected intravenously with 2×1011 VG of scAAV9/MeP-GFP, and then killed 14 weeks postinjection to assess GFP-positive cells in the CNS. (A–C) IHC using an anti-GFP antibody in the hippocampus (A), striatum (B), and spinal cord (C). The inset in (B) shows an additional ×5 magnification. Rows D–F show alternate hippocampal (D), striatal (E), and spinal cord (F) sections prepared in parallel with no primary antibody. Rows G–I are representative confocal images showing co-IF with GFP and NeuN antibodies in the hippocampus, striatum, and spinal cord, respectively. Scale bars: 200 μm.