(a) Gαq-V fluorescence recovery after photobleaching in cells expressing C-TM (n=22), C-M3R in the presence of 10 μM atropine (n=44) or C-M3R in the presence of 50 μM carbachol (n=42). (b) Unrecovered fluorescence for the same cells as panel a; **, P<0.001 compared to C-TM. (c) Gαq-V fluorescence recovery in permeabilized, nucleotide-depleted cells expressing C-M3R in the presence of atropine (n=15), carbachol (n=15), or in the presence of carbachol with 0.3 mM GTPγS (n=15) or 0.3 mM GDPβS (n=24). (d) Unrecovered fluorescence for the same cells as panel c; **, P<0.001. (e) Gαq-V fluorescence recovery in permeabilized, nucleotide-depleted cells expressing C-M3R in the presence of atropine (n=20), carbachol (n=20), or in the presence of carbachol with atropine added at 100 seconds (arrow; n=20). Data represent mean values ± s.e.m..