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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Nat Chem Biol. 2011 Aug 28;7(10):740–747. doi: 10.1038/nchembio.642

Figure 3. The M3R C terminus is necessary and sufficient for preassembly with Gq.

Figure 3

(a) Gαq-V fluorescence recovery in cells expressing C-TM (n=30), C-M3R (n=30), a chimeric C-M3R with the C terminus of M4R (C-M3R(M4ct); n=30) or C-M4R (n=24). (b) Unrecovered fluorescence for the same cells as panel a; **, P<0.001 compared to C-TM. (c) Gαq-V fluorescence recovery in cells expressing C-TM (n=16), C-M3R (n=18), a chimeric C-M4R with the C terminus of M3R (C-M4R(M3ct); n=37) or C-M4R (n=30). (d) Unrecovered fluorescence for the same cells as panel c; **, P<0.001 compared to C-TM. Unrecovered fluorescence for C-M3R and C-M4R(M3ct) were not significantly different (P=0.14). Data represent mean values ± s.e.m..