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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Nat Chem Biol. 2011 Aug 28;7(10):740–747. doi: 10.1038/nchembio.642

Figure 4. A polybasic region in the M3R C terminus is necessary for preassembly with Gq.

Figure 4

(a) Alignment of the M4R and M3R C termini, together with mutants where amino acids 565–570 are replaced with alanines (6A) or have lysines and arginines exchanged for each other (RRRKKR). (b) Gαq-V fluorescence recovery in cells expressing C-TM (n=43), C-M3R (n=43), C-M3R(6A) (n=43) or C-M3R(RRRKKR) (n=20). (c) Unrecovered fluorescence for the same cells as panel b; **, P<0.001; *, P=0.047 compared to C-TM. Data represent mean values ± s.e.m..