Fig. 1.
ORF57 binds the 5′ end of PAN RNA in lytically reactivated cells. (A) Schematic diagram of PAN RNA with approximate positions of qRT-PCR amplicons shown below. Primer set A, B, C, and D amplify PAN RNA base pairs 50–124, 293–372, 642–728, and 994–1064, respectively. The numbering system for PAN RNA in this manuscript is relative to the start site as defined in Zhong et al. (1996). (B) Results from UV cross-linking immunoprecipitation experiments. The y-axis shows the immunoprecipitation efficiencies relative to that for primer set A (see Materials and Methods). The error bars are standard deviation (n=3).