Fig. 4.

In vitro expansion of Vγ9 Vδ2 T cells from RCC patients in response to ZA and IL-2. PBMC from Patients 1–4 and 7 in Cohort A were cultured with the indicated concentration of ZA in the presence of 200 U/ml IL-2. Absolute numbers of viable Vγ9 Vδ2 T cells were calculated by flow cytometry after 7 days in culture. Graphs show the expansion of Vγ9 Vδ2 T cells from Patients 1–4 and 7, respectively. Expansion indices indicate the ratio between Vγ9 Vδ2 T cells on Day 7 and the initial number of Vγ9 Vδ2 T cells seeded in culture