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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Clin Biochem. 2011 Jul 6;44(14-15):1241–1246. doi: 10.1016/j.clinbiochem.2011.06.983

Fig. 6. Simultaneous detection of cytokine (IL-10) and antibody (anti-PGL-I IgM).

Fig. 6

Seven serum samples from leprosy patients spiked with IL-10 (1000 pg/mL). The five multibacillary leprosy patient’s sera, positive in ELISA for anti-PGL-I IgM antibodies, also showed positive signals in the UCP-LF for anti-PGL-I. Two paucibacillary leprosy patients’ sera that were negative for anti-PGL-I IgM antibodies in ELISA as well as a healthy control serum sample, did not show a relevant signal with the UCP-LF assay. UCP-LF IL-10 signals were detected in all spiked samples; the variability in signal can be partly attributed to unidentified IL-10-complexing components in the individual sera.