Fig. 1.
Bone marrow was collected from Anaplasma phagocytophilum-infected and uninfected mice on days 0–21 after tick-borne infection. An aliquot of BM was plated in duplicate and cultured in complete MethoCult media for 7–10 days (StemCell Technologies) according to the manufacturer’s instructions. The colonies were counted and scored as granulocytic/monocytic, mixed or erythroid. Depicted are representative images of assays on day 7 after A. phagocytophilum infection. (a) Infected mouse, low power. (b) Uninfected mouse, low power. (c) Infected mouse with increased macrophages, high power. (d) Uninfected mouse, high power. (e) Infected mouse, high power. (f) Uninfected mice infested with ticks, high power.