Fig. 2.

Immunoblot showing that MMP20 cleaves the E-cadherin extracellular domain. The recombinant human E-cadherin/Fc chimera that links the E-cadherin extracellular domain to an Fc fragment was incubated with MMP20 that was purified from porcine enamel. Incubations were for 0 or 20 h followed by immunoblotting with primary antisera specific for E-cadherin or the linked Fc fragment. The secondary antiserum was from rabbit (for IgG-Fc Ab) or goat (for E-Cadherin Ab). Also shown is an SDS-PAGE gel stained for protein and 2 negative control immunoblots that were treated with only the secondary antisera.