Fig. 3.

QRT-PCR analyses of type I collagen (a), type II collagen (b), runx2 (c), and BSP (d) from frozen-ground specimens retrieved from nude mice after 10 and 20 weeks of implantation. Statistically significant differences (p < 0.1) are designated by an asterisk (∗) between given gene expression levels for the different constructs sutured with periosteum. Forward and reverse primers for bovine-specific type II collagen and BSP have been published [Kusuhara et al., 2009]. Bovine-specific primers for runx2, type I collagen, and cyclophilin D are as follows: