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. 2011 May 20;25(8):1074–1082. doi: 10.1038/eye.2011.109

Figure 1.

Figure 1

Verification of sub-lytic MAC assembly. (a) MTT viability assay measuring the amount of cell death after treating ARPE-19 cells with anti-CD59, and ascending concentrations of NHS for 1 h at 37 °C. For negative controls, cells were treated with anti-CD59/HI–NHS, and for positive controls with 1% Triton X-100. Data are plotted as means±s.d. (n=5). (b) The mean fluorescence intensity of C5b-9 using flow cytometry presented as a histogram in control (anti-CD59/HI–NHS; open histogram), and anti-CD59/NHS-treated cells (grey histogram) (n=4 for each treatment). (c) Representative images (n=4) from immunofluorescence staining for C5b-9 after MAC assembly on ARPE-19 cells. 1=HI–NHS alone, 2=NHS alone, 3=anti-CD59/HI–NHS, 4=anti-CD59/NHS. The scale bar indicates 10 μm.