FIG. 3.

Effects of organic nitrate treatment on cardiac and serum oxidative stress in diabetic rats. A: Quantification of cardiac NADPH oxidase activity in membranous preparations by ECL using the superoxide-specific dye, lucigenin (5 µmol/L). B: Quantification of serum XO activity by superoxide-dependent cytochrome c (50 µmol/L) reduction traced at 550 nm. C: Detection of serum antioxidant capacity by low-molecular weight antioxidant-dependent 2,2-diphenyl-1-picrylhydrazyl radical (DPPH^•) (50 µmol/L) reduction traced at 517 nm. Serum was deproteinized by the addition of 50% acetonitrile. Data are the means ± SEM of heart tissues and serum samples from 10–20 (A), 6–10 (B), and 6–12 (C) animals per group. *P < 0.05 vs. control; #P < 0.05 vs. STZ.