FIG. 4.

Pancreas and islet histomorphometry in RIP-CA-PKC-ζ mice. Histomorphometry was performed in insulin-stained pancreatic sections from 12- to 14-week-old WT (n = 11) and TG (n = 9) littermates and β-cell mass (A), pancreas weight (B), islet number per pancreatic area (C), and number of single and double insulin-positive cells embedded in the acinar tissue and in ductal structures per pancreatic area (D) were measured. β-Cell mass was significantly increased in TG mice with no alteration in pancreas weight, islet number, or small clusters of insulin-positive cells. Results are means ± SEM. *P < 0.05 vs. WT. E: Representative microphotographs of WT and TG mouse pancreatic sections stained for insulin and counterstained with hematoxylin. TG mouse islets were increased in size compared with WT mice. F: Histomorphometric analysis of the islet size distribution per pancreatic area in pancreatic sections from WT and TG mice showed a significant increase in the number of islets with a diameter larger than 100 μm (larger islets) in TG mice. Results are means ± SEM and *P < 0.05 vs. WT of the same islet size. (A high-quality color representation of this figure is available in the online issue.)