FIG. 5.
Adipocytes become more chemoattractive to macrophages after 3 days of HFD. A: Macrophage migration assays using primary ACM from mice treated with NCD (N) or HFD. ACM were obtained by incubating mouse primary adipocytes in serum-free Dulbecco’s modified Eagle’s medium for 12 h. 3d, 3 days; 1w, 1 week. B: MCP-1 protein expression from the primary adipocytes. C: MCP-1 protein secretion from those adipocytes. *P < 0.05; **P < 0.01.