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. 2011 Sep 16;60(10):2578–2587. doi: 10.2337/db11-0058

FIG. 6.

FIG. 6.

A–F: Hyperinsulinemic-euglycemic clamp in wild-type (Wt) and A1AR−/− mice. Blood glucose (A), glucose infusion rate (GIR) (B), plasma glucose (C), plasma insulin (D), whole-body glucose fluxes (E), and tissue 2-DG (F) uptake were measured in 5-h–fasted, 18-week-old Wt (n = 16) and A1AR−/− (n = 14) littermate mice on a C57BL/6 background during a hyperinsulinemic-euglycemic clamp. C–E: Basal parameters are the means ± SEM of data collected at −30 and −5 min (after a 90-min equilibration). At 0 min, mice were infused with insulin (4 mU/kg/min) and 20% glucose (variable rate) to maintain blood glucose concentrations at ~150 mg/dL. E: Clamp whole-body fluxes are the means ± SEM of data collected at 90–120 min. D: Clamp insulin was measured at 110 min. C: Bolus of 2-DG was administered at 80 min, and mice were killed at 120 min for tissue processing. BAT, brown adipose tissue; EGP, endogenous glucose production; Muscle, gastrocnemius muscle; RD, glucose disposal rate. *P < 0.05; **P < 0.01.