Table 2. Primer pairs used in this survey.

Sequence/reference		T	a.r.	a.p.
5S-Univ-F	Vierna et al. (2009)	50 °C	5S	13–32
5S-Univ-R	Vierna et al. (2009)	50 °C	5S	36–55
5S-U1-F	5′ GTCTACGGCCATATCACGTT	61 °C	5S	1–20
5S-U1-R	5′ GTTAGCGCGAACGCAGVC	61 °C	U1	142–159
U1-5S-F	5′ VCTGCGTTCGCGCTAVCC	65 °C	U1	143–160
U1-5S-R	5′ GGTATTCCCAGGCGGTCAC	65 °C	5S	87–105
U1-U1-F	5′ GCAATGGAAGGGCCTCCTCCT	61 °C	U1	49–69
U1-U1-R	5′ TTCGGTTGGGCTGATGCCTG	61 °C	U1	72–91

Abbreviations: a.p., annealing position within each RNA coding region; a.r., annealing region; T, annealing temperature; U1, U1 small nuclear RNA coding region; 5S, 5S ribosomal RNA coding region.