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. 2011 Mar 2;107(2):127–142. doi: 10.1038/hdy.2010.174

Table 2. Primer pairs used in this survey.

Sequence/reference T a.r. a.p.
5S-Univ-F Vierna et al. (2009) 50 °C 5S 13–32
5S-Univ-R Vierna et al. (2009) 50 °C 5S 36–55
         
5S-U1-F 5′ GTCTACGGCCATATCACGTT 61 °C 5S 1–20
5S-U1-R 5′ GTTAGCGCGAACGCAGVC 61 °C U1 142–159
         
U1-5S-F 5′ VCTGCGTTCGCGCTAVCC 65 °C U1 143–160
U1-5S-R 5′ GGTATTCCCAGGCGGTCAC 65 °C 5S 87–105
         
U1-U1-F 5′ GCAATGGAAGGGCCTCCTCCT 61 °C U1 49–69
U1-U1-R 5′ TTCGGTTGGGCTGATGCCTG 61 °C U1 72–91

Abbreviations: a.p., annealing position within each RNA coding region; a.r., annealing region; T, annealing temperature; U1, U1 small nuclear RNA coding region; 5S, 5S ribosomal RNA coding region.