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. Author manuscript; available in PMC: 2011 Sep 22.
Published in final edited form as: Cell Immunol. 2007 May 3;245(1):1–6. doi: 10.1016/j.cellimm.2007.03.006

Fig. 4.

Fig. 4

Effect of cell stimulation and GlcN addition on cell metabolism. (A) ATP levels of Jurkat cells were measured using the ATPlite kit as directed by the manufacturer. Luminescence was measured using a Molecular Devices FlexStation II. No statistically significant differences were found. (Raw data in counts are shown.) (n=3) Effect of GlcN on Jurkat NAD(P)H (B) and flavoprotein (C) autofluorescence. Experiments were conducted at 37°C. Jurkat cells were placed on Cell-Tak-coated coverslips, to allow addition of GlcN without disturbing the cells. GlcN (40 mM) was added at the arrowheads in both panels. A decrease in NADH was observed, followed by slow recovery over time. Similar changes in flavoprotein autofluorescence were found. A PTI photometer using a low noise LED at 365nm for excitation was used for NADH and a conventional Hg lamp at 460nm for the flavoprotein excitation. (n=3) (Unprocessed data are shown.)