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. Author manuscript; available in PMC: 2011 Sep 22.
Published in final edited form as: Dev Biol. 2008 Apr 25;319(2):248–257. doi: 10.1016/j.ydbio.2008.04.019

Figure 3.

Figure 3

Notch is necessary and sufficient to restrict lactotrope differentiation in the anterior pituitary placode. (A,B) Wild-type embryos labeled with lim3 (A, n=85) and prl (B, n=96). (B, inset) Bilateral cells in the anterior pituitary placode express prl. Gene expression in DMSO treated (n=30), and hsp70:Gal4 heat-shocked (n=30) control embryos was similar to wild-type embryos in A and B. (A,C,E,G) Pituitary placode size and lim3 expression in mib mutant (C, n=39), in DAPT treated (E, n=45), and in heat shocked hsp70:Gal4 × UAS:notch1a (G, n=22) transgenic embryos is similar to controls (A,B). (B,D,F) Loss of functional Notch signaling in mib mutant (D, inset, n=37/37) and DAPT treated (F, inset, n=32/40) embryos leads to expansion of prl in pituitary placode (arrowheads; D,F). (H) prl expression in the anterior pituitary placode is completely lost in hsp70:Gal4 × UAS:notch1a transgenic embryos (n=48/48) following heat shock. (A–G) side views, anterior to the left, dorsal to the top; (A–G, inset) frontal views, dorsal to the top. All embryos Prim-5 (24h) stage. Scale bar: 50 µm (A–H); Insets A,C,E,G: 40 µm; Insets B,D,F,H: 30 µm.