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. Author manuscript; available in PMC: 2011 Sep 22.
Published in final edited form as: Dev Biol. 2008 Apr 25;319(2):248–257. doi: 10.1016/j.ydbio.2008.04.019

Figure 5.

Figure 5

Gain of Notch function increases the number of pomc expressing corticotropes and melanotropes at the expense of lactotropes and thyrotropes. Embryos carrying either the hsp70:Gal4 or the hsp70:Gal4 × UAS:notch1a transgenes were heat shocked at Bud (10h) stage and analyzed for hormone expression at Protruding mouth (72h) stage (A–J). In heat-shocked hsp70:Gal4 × UAS:notch1a transgenic embryos, both prl (F, n= 36/36) and tsh-β (I, n=42/42) are completely lost, pomc expression is expanded in APD, PPD and PI (G, n=32/32), and cga is expanded (J, n=15/17). gh expression is unaffected (H, n=40/40) compared to prl (A, n=34) and tsh-β (D, n=32), pomc (B, n=37), gh (C, n=30) and cga (E, n=20) in hsp70:Gal4 embryos following heat shock. Dotted lines indicate outline of anterior pituitary tissue (omitted in panels where pituitary border was ambiguous). (A–J) Ventral views anterior to the top. Scale bar: 15 µm (A–J)