Table 2.
Notch Signal is necessary between 12h and 15h for pituitary cell specification
| Hormone expression at 72h compared to DMSO treated control embryos | |||||
|---|---|---|---|---|---|
| DAPT treatment |
Hormone | Expanded (%) |
Reduced (%) | Lost (%) |
Total Embryos |
| 6h – 72h | pomc anterior | - | - | 96 | 25 |
| prl | 78 | - | - | 45 | |
| gh | - | - | 76 | 63 | |
| tsh-β | 73 | - | - | 40 | |
| cga | 80 | - | - | 25 | |
| pomc posterior | - | 92 | - | 25 | |
| 10h – 72h | pomc anterior | - | - | 92 | 25 |
| prl | 77 | - | - | 60 | |
| gh | - | - | 75 | 60 | |
| tsh-β | 80 | - | - | 60 | |
| cga | 76 | - | - | 50 | |
| pomc posterior | - | 84 | - | 25 | |
| 12h – 72h | pomc anterior | - | - | 88 | 25 |
| prl | 84 | - | - | 44 | |
| gh | - | - | 77 | 30 | |
| tsh-β | 80 | - | - | 30 | |
| cga | 83 | - | - | 30 | |
| pomc posterior | - | 84 | - | 25 | |
| 16h – 72h | pomc anterior | - | - | - | 20 |
| prl | 2 | - | - | 55 | |
| gh | - | - | - | 32 | |
| tsh-β | 4 | - | - | 52 | |
| cga | 2 | - | - | 50 | |
| pomc posterior | - | - | - | 20 | |
| 6h – 40h | pomc anterior | - | - | 96 | 25 |
| prl | 82 | - | - | 34 | |
| gh | - | - | 76 | 29 | |
| tsh-β | 84 | - | - | 38 | |
| cga | 75 | - | - | 40 | |
| pomc posterior | - | 84 | - | 25 | |
DAPT treatment affects hormone expression in Protruding mouth (72h) stage embryos. The frequency of change in hormone gene expression domain was scored as expanded (as observed in Fig. 4K,N,O), reduced (as observed in Fig. 4L), or lost (as observed in Fig. 4M).