. Author manuscript; available in PMC: 2011 Sep 22.

Published in final edited form as: Dev Biol. 2008 Apr 25;319(2):248–257. doi: 10.1016/j.ydbio.2008.04.019

Table 3.

Overexpression of NICD between 10h and 24h, but not after 36h, affects pituitary hormone expression

	Hormone expression in Tg(hsp70:GAL4) × Tg(UAS:Notch1a) embryos compared to Tg(hsp70:GAL4) control embryos

Heat-Shock at	Hormone	Expanded (%)	Reduced (%)	Lost (%)	Total Embryos
10h	pomc anterior	95	-	-	25
	prl	-	-	98	89
	gh	-	-	-	68
	tsh-β	-	-	100	57
	cga	90	-	-	30
	pomc posterior	95	-	-	25

18h	pomc anterior	93	-	-	30
	prl	-	-	100	80
	gh	-	-	-	38
	tsh-β	-	-	100	50
	cga	100	-	-	40
	pomc posterior	93	-	-	30

24h	pomc anterior	90	-	-	40
	prl	-	-	96	25
	gh	-	-	-	25
	tsh-β	-	-	96	25
	cga	90	-	-	20
	pomc posterior	90	-	-	40

36h	pomc anterior	-	-	-	50
	prl	-	-	-	55
	gh	-	-	-	55
	tsh-β	-	-	-	50
	cga	-	-	-	50
	pomc posterior	-	-	-	55

Notch activation regulates expression of all hormone producing cell types at Protruding mouth (72h) stage, except somatotropes. The frequency of change in hormone gene expression domains was scored as expanded, as observed in Fig. 5G; lost, as observed in Fig. 5F,I.