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. Author manuscript; available in PMC: 2011 Sep 22.
Published in final edited form as: Nature. 2010 Mar 18;464(7287):427–430. doi: 10.1038/nature08902

Figure 1. RAF inhibitors rapidly activate MEK/ERK in cells with wild-type BRAF.

Figure 1

a, Calu-6 cells (BRAFwild-type/K-RASQ61K) were treated with increasing doses of the indicated RAF inhibitors and the effects on ERK signaling were determined by immunoblotting for pMEK and pERK. b, Cells with wild-type BRAF (Calu-6) or mutant BRAF (Malme-3M) were treated with vehicle or PLX4720 (1μM/1 hour). Phosphorylation and expression of the indicated proteins were assayed by immunoblotting. c, Calu-6 cells treated with 1μM PLX4720 for the indicated time points. d, Calu-6 cells were treated with 1μM PLX4720 for 60 minutes, then medium was replaced with medium containing 1μM PLX4720 (lanes 3-5) or vehicle (lanes 8-10) for the indicated time points.