A: Mice with established HCT116 KRAS mutant/PIK3CA wild-type (wt PIK3CA, red) and HCT116 KRAS mutant/PIK3CA mutant (mut PIK3CA, blue) xenografts were treated with 5mg/kg of PD0325901 or vehicle only, 5 days/week for the indicated number of days. The results are represented as in Figure 2D.
B and C: Immunoblots of homogenized xenograft tissue after a single 5mg/kg dose of PD0325901. Tumors were excised pretreatment and at indicated times and split in half; (B) one half was flash frozen for immunoblot analysis with indicated antibodies; (C) the other half was formalin fixed, paraffin embedded and used for tissue-section preparation. 8 µm tissue sections were cut and stained with human specific antibody for cleaved PARP.