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SIMPLE is widely expressed in several tissues and is highly expressed in the peripheral nerves and Schwann cells. (A,B) Equal amounts of total proteins (100 μg protein/lane) from the indicated mouse tissues (A) or primary Schwann cells and cortical neurons (B) were subjected to immunoblot analysis with anti-SIMPLE and anti-β-actin antibodies. (C) Primary Schwann cells immunostained with antibodies against S-100 or SIMPLE. Nuclei are visualized using DAPI stain. Scale bars: 10 μm.
