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Endogenous SIMPLE is localized to the early endosome but not to other organelles. HeLa cells were double-immunostained with antibodies against endogenous SIMPLE (green) and EEA1, LAMP2, GM130 or KDEL (red). Colocalization is indicated by the yellow color in the merge panel, and examples are indicated by arrowheads. Scale bars: 10 μm.
