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. 2011 Oct 1;124(19):3319–3331. doi: 10.1242/jcs.087114

Fig. 7.

Fig. 7.

CMT1C-associated SIMPLE mutants are accumulated in aggresomes. (A) Stably transfected HEK293 cells expressing Myc-tagged WT, W116G or P135T SIMPLE were treated for 24 hours with 2 μM MG132 or vehicle control (CTRL) and then immunostained with anti-Myc antibody (red). Nuclei were visualized by DAPI stain. Aggresomes are indicated by open arrowheads. Scale bars: 10 μm. (B) The percentage of cells containing SIMPLE-positive aggresomes was quantified and shown as mean ± s.e.m. from three independent experiments. *P<0.05 compared with SIMPLE WT under the same treatment. (C) HEK293 cells expressing Myc-tagged P135T SIMPLE were treated with 2 μM MG132 for 24 hours and then double-immunostained with antibodies against the Myc tag (red) and LC3 or LAMP2 (green). Aggresomes are indicated by open arrowheads. Scale bars: 10 μm.